Posted byGenetics|Bioinformatics|Industry4 years ago
Archived
I absolutely positively hate this kit. I have never had recovery beyond 1 to 5% of my plasmid or gene in question.
Quiagen Gel purification. How I hate thee (self.labrats) submitted 4 years ago by Moklomi Genetics. Sadly No after debating with the quiagen help line they sent us a new one. I have done many many cloning experiments using this kit for gel purification. It might be more on the expensive side, but boy is it ever worth it.
I need tips !
Protocol for reference: Start with 9 ug of digested Plasmid run on a wide comb .8% gel of low melt agrose and cut on an invitrogen safe imager.
Measure tube before hand and after never add more than 400g of agrose to one tube.
Add QG 5 Gel volumes.
Melt 5 mins at 55C vortexing every 30 seconds
Add 100% 2 propanal in one to one volume of gel.
Add to column and spin.
Add PE at 750ul ice cold and kept at -20*c
Wash via spin
Dry column with another wash.
Add 60*c EB to center of column wait 5 mins and spin.